To develop innovative drug delivery carriers for controllable release and cancer-targeted delivery of therapeutic agents to accomplish efficient cancer chemotherapy.Herein we effectively fabricated CaCO3 primarily loaded biotin (BT) and directly the self-assembly of oxaliplatin (Pt (IV)) prodrugs form in liposomes.The acquired BT-Pt (IV)@PEG/CaCO3 with outstanding biological stability displays rapid pH-mediated degradations, thus allowing the effective pH-responsive delivery of BT.In vitro, anticancer assays proved that BT-Pt (IV)@PEG/CaCO3 effectively kills Figurine the thyroid cancer cells (B-CPAP and FTC-133).The biochemical staining assays investigated the morphological changes of thyroid cancer after treatment with nanoparticles.
The DNA fragmentation of the cells was assessed by utilizing the comet assay.BT-Pt (IV)@PEG/CaCO3 increased ROS levels Measuring Spoon Sets and caused mitochondrial membrane potential and DNA damage, which resulted in apoptosis.Due to its versatile drug-loading capability, this research demonstrates that CaCO3 liposomal formulation is a biocompatible and reliable substrate for establishing pH-mediated drug delivery methods and promising for possible therapeutic application.